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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was uncovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were being described but thorough nucleotide sequence info will not be but available.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This guidebook outlines further more feasible optimization of mobile and immunoassay parameters to be sure the very best outcomes are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead on the temporal coordination of each and every mitotic celebration. Cyclin E varieties a posh with and functions being a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is particularly degraded as cells development through S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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